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OBJECTIVE To improve the anticancer drug gambogic acid’s effect by using titanium dioxide coated gold nanorods(GNR/Ti O2)as a drug carrier.METHODS Biocompatibility and cellular uptake of GNR/Ti O2was studied in human glioblastoma U-87 MG cells.Cel viability was evaluated by ATP assay and calcein AM staining.Lyso SensorTMGreen DND-189 and Hoechst 33342 were used to analyze the intracellular location of GNR/Ti O2.The in vitro anticancer effect of gambogic acid loaded nanoparticles was compared with free drug.RESULTS The results showed that GNR/Ti O2is biocompatible,andthey are localized at the intracellular acidic compartments of endosomes and lysosomes.The intracellular drug content delivered via GNR/Ti O2was 6 fold higher than the free form,thus dramatically enhancing the anticancer effect of gambogic acid.Furthermore,mild photothermal therapy also showed synergistic effect with the drug.CONCLUSION Our study suggested that GNR/Ti O2is a promising anticancer drug carrier。
OBJECTIVE To improve the anticancer drug gambogic acid’s effect by using titanium dioxide coated gold nanorods (GNR / Ti O2) as a drug carrier. METHODS Biocompatibility and cellular uptake of GNR / Ti O2was studied in human glioblastoma U-87 MG cells. Cel viability was evaluated by ATP assay and calcein AM staining. Lyso Sensor TM Green DND-189 and Hoechst 33342 were used to analyze the intracellular location of GNR / Ti O2. in vitro anticancer effect of gambogic acid loaded nanoparticles was compared with free drug. that GNR / Ti O2is biocompatible, and they are localized at the intracellular acidic compartments of endosomes and lysosomes. The intracellular drug content delivered via GNR / Ti O2was 6 fold higher than the free form, thereby enhancing the anticancer effect of gambogic acid. mild photothermal therapy also showed synergistic effect with the drug. CONCLUSION Our study suggested that GNR / Ti O2is a promising anticancer drug carrier.