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Aspergillus niger,a species of filamentous fungi,is universally acknowledged of having long been investigated for industry production of metabolites and enzymes.Meanwhile,with the growth of the biotechnology industry,it has been gradually employed in areas as diverse as medicine,agriculture,and basic science that influence our everyday lives.Taking advance of the expression system in Aspergillus niger,our team successfully accomplished “Visual operation system of continuous gene replacement” and “From Red to Cyan”,respectively means targeted genes location at specific sites and transformation accelerator from lycopene to astaxanthin.The meaning of our work,on one hand,relied on that we established a new visual operation system assisting us to make sure where is the DNA.On the other hand,we designed one/two fusion protein contributing to the transformation accelerator of metabolism.