【摘 要】
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We have developed a high throughput multiplex pan-arbovirus PCR diagnostic platform for surveying East African mosquito and tick populations.We employ an emerging Mass Spectrometry based diagnostic pl
【机 构】
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icipeP.O. Box 30772-00100, Nairobi, Kenya
【出 处】
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第二届国际蚊虫及蚊媒病监测和防治学术研讨会
论文部分内容阅读
We have developed a high throughput multiplex pan-arbovirus PCR diagnostic platform for surveying East African mosquito and tick populations.We employ an emerging Mass Spectrometry based diagnostic platform that can identify up to 30 target PCR amplicons labeled with distinct MassTags.We have designed MassTag PCR panels with universal primers for alphavirus,Sindbis,phlebovirus,nairovirus,orthobunyavirus,flavivirus and Thogoto.In second tier MassTag PCR reactions we can screen for specific arboviruses.
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