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The purpose of this study wasto develop a yield prediction model by measuring amount of nutrient uptake in plant factory for hydroponically grown lettuce.An automated system was developed to control pH and Electrical Conductivity (EC) of nutrient solution and measuring the amount of nutrient absorbed.The integralnutrient uptake in full day, light period and dark period were compared with fresh mass of lettuce after harvested.The result shown the integral nutrient uptake in full day had best linear relationship (R2 : 0.95) with the fresh mass harvested.This system can predict the fresh mass of lettuce harvested after 3 days of pure water treatment for nitrate control, and can also be used as early warning mechanism when the nutrient absorbed are low compare with normal conditions.