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Aim: To develop a novel, simple and accurate fingerprint method for the quality control of Clerodendranthus Spicatus from different regions by high-performance liquid chromatography, which is widely used for urolithiasis.Methods: Chromatographic separation was performed on Agilent SB-C18 (250 mm×4.6 mm, 5 μm) by the gradient elution of 0.1% H3PO4 in water and acetonitrile at 0.8 mL·min-1 at 210 nm by DAD detector.The column temperature was set at 30 ℃.Results: The method has good stability and reproducibility with RSD less than 2%.10 peaks were selected as the common peaks.Rosmarinic acid was taken as a reference.The similarity of 10 batches was above 0.90.Conclusion: This method is simple and specific, which provided an effective way to assess the quality of Clerodendranthus Spicatus.