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Pteris multifida is a triditional fern in China, with overlapping distribution area with a main invasive plant Bidens pilosa.Root exudates, as B.pilosa and non invasive plant Rudbeckia hirta, were applied to investigate their effects on fluorescence in P.multifida gametophytes.The result showed that gametophytes exposed to both B.pilosa and R.hirta had decreased fluorescence parameters in comparison with the control, except for non-photochemical quenching (NPQ).The inhibitory effects turned strong with increasing exudate concentrations of both species.And the greatest inhibition exhibited at Day 10.In addition, it was found that these parameters were markedly affected from Day 4 to Day 10 in the presence of both exudates at a concentration of 12.5% or above.It is concluded that both B.pilosa and R.hirta negatively affected the photosynthetic activity of P.multifida.Comparative research of invasive and non-invasive plants in our experiments illustrated that there was critical damage to local species under situation of invasive plants Bidens pilosa.