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The natural biomaterial sarcosine as the electron transport layer (ETL) to modify ITO or ITO/ZnO was successfully introduced into the inverted OSCs with PM6∶BTP-BO-4Cl as the active layers.The introduction of sarcosine on the surface of ITO or ITO/ZnO can result in lower work function (WF) and higher surface energy.The active layers processed on the surfaces of ITO or ITO/ZnO have more optimized morphology and more ordered molecular arrangement after modifying with sarcosine.The introduction of sarcosine as ETL can promote charge transport and collection in the OSCs.Therefore,the PCE of OSCs is increased to 13.53% from 3.86% by modifying ITO with sarcosine.The PCE of OSCs with ZnO as ETLs is improved to 16.45% from 14.85% by modifying ZnO with sarcosine.The improved PCEs are benefited from the simultaneously improved short-circuit current density (JSC),fill factor (FF),and open-circuit voltage (VOC).This work demonstrates that sarcosine has great potential as an ETL to improve the performance of OSCs.