【摘 要】
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Brucella is the etiological pathogen of Brucellosis,a zoonosis characterized by chronic infection in human.AsnC is a member of Lrp/AsnC type treanscriptional regulator that exists in various eukaryoti
【机 构】
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Key Laboratory of Zoonotic of Liaoning Province,College of Animal Science and Veterinary Medicine,Sh
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Brucella is the etiological pathogen of Brucellosis,a zoonosis characterized by chronic infection in human.AsnC is a member of Lrp/AsnC type treanscriptional regulator that exists in various eukaryotic bacteria.Our previous protective antigen screening study showed that immunization of AsnC could induce protective immunity against virulence strain challenge,implying that it is a good candidate for subunit vaccine development.In the present study,we investigated the involvement of AsnC in virulence of B.melitensis.The expression profiles of AsnC in vitro and during infection were firstly analyzed.AsnC is induced under in vitro stresses that simulating intracellular environment.Compared with that in vitro,expression of asnC was induced during macrophage infection,particularly during late infection phase.Mouse infection assay showed that asnC mutant showed identical survival capabilities during early infection stage but reduced capability in chronic stage.Expression of several virulence genes was reduced in asnC mutant when compared with that in wild type strain,indicating that asnC affect expression of other virulence genes during infection.Taken together,AsnC is induced in infection related conditions and involved in virulence regulation during chronic infection of B.melitensis.
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