Oligodeoxynucleotides with Novel Benzocoumarin-triazine Conjugates Labeled at the Middle Position of

• 作 者: Min-Rui Liu Jia-Chun Liu Min Chen Wen Zhang
• 机 构: Lab of Chemical Biology and Design of Molecular Drugs,College of Pharmaceutical Science,Zhejiang Uni
• 出 处: 中国化学会第29届学术年会
• 发表日期: 2014年1期
• 关键词: Benzocoumarin-triazine DNA probe DNA hybridization Fluorescence enhancement

　　The design and synthesis of fluorescence-labeled oligonucleotides that exhibit significant enhanced emission on hybridization to complementary nucleic acid sequences have been the subject of intense research due to their application in the detection of specific nucleic acids in solution.Solution hybridization could simplify the detection procedures and give the possibility for use in vivo.Because of having big planar and rigid structure,not only are coumarin derivatives excellent DNA intercalators,but also are important fluorescent fluorophores which could be applied to laser dyes and cell fluorescent probes.Thus,we have been interested in the development of a benzocoumarin derivative-labeled DNA fluorescent probe possessing the property whereby its binding to a DNA sequence could result in the fluorescence increase in homogeneous solution.Here,we describe the design,synthesis and application of three benzocoumarin-triazine-labeled DNA hybridization probes(Fig.1)in homogeneous solution,of which the fluorophore labeled at middle position of an oligodeoxynucleotide shows unusual fluorescence enhancement over that at terminal on hybridization.The hybridization to complementary DNA results show that the fluorescence intensity of the angel parent compounds modified by substituted 1,3,5-triazine in buffer increased significantly,indicating the potential application of the compound as a DNA fluorescent probe.