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Honeybee venom is a complicated defensive insect toxin that has a wide range of pharmacological applications for humantherapeutics.Manual (or reservoir disrupting) venom extraction from the venom gland (GV) and electrical stimulation (ESV) arethe two major methods of obtaining honeybee venom.However,the composition of these two venom forms is still lacking a fullcharacterization.The proteome of GV and ESV were compared using gel-based (one-dimensional gel electrophoresis,twodimensionalgel electrophoresis) and gel-free proteomics approaches.