Analysis of Polychlorinated Biphenyls Congeners in Soil Samples by Real-time Immuno-PCR assay

• 作 者: Chen Han-yu Zhuang Hui-sheng
• 机 构: SchoolofEnvironmentalScienceandEngineering,ShanghaiJiaoTongUniversity,Shanghai,200240,China;Institut
• 出 处: 中国化学会第十七届全国有机分析与生物分析学术研讨会
• 发表日期: 2013年10期
• 关键词: 3 4-dichlorinated biphenyl Polyclonal antibody Real-time immuno-PCR

　　A novel real-time immuno-PCR (RT-IPCR) assay for the determination of 3,4-dichlorinated biphenyl (IUPAC PCB12) is described in this paper.Using the optimized assay,the linear range for the determination of PCB12 was found to be 10.0 fg mL-1 to 1.0 ng mL-1 with a correlation coefficient of 0.987 and a detection limit of 1.53 fg mL-1.The RT-IPCR assays were tested for their cross-reactivity profiles using four selected congeners and four Aroclor products.The assays were highly specific for the congeners; however,they were less specific for Aroclor 1242.The RT-IPCR results for the soil samples correlated with the concentrations of PCBs obtained by gas chromatography/mass spectrometry.