Single Emx1-expressing neural stem cells give rise to both projection neurons and interneurons in vi

• 作 者: Yu-QunCAI;YueZHANG;Zhen-GangYANG;
• 机 构: Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shan
• 出 处: 中国神经科学学会第九届全国学术会议暨第五届会员代表大会
• 发表日期: 2011年期

　　There are two major subtypes of neurons in the brain: projection neurons and interneurons.During Drosophila nervous system development, a subpopulation of single neuroblasts (neural stem cells) can give rise to both projection neurons and interneurons.However, in mammalian brains, whether single neuroepithelial cells or radial glias (primary neural stem cells) can produce both projection neurons and interneurons remains largely unknown.Using fluorescence-activated cell sorting (FACS), we cultured single Emxl-expressing neural stem cells derived from El 0.5-11.5 mouse cortex.Two weeks after culturing in defined serum-free medium, multiple-immunostaining revealed that a subpopulation of single Emxl-expressing neural stem cells can produce both projection neurons and interneurons.The projection neurons in a single cell-derived clone express typical projection neuronal markers, such as Tbr1, Cux1, Ctip2, Foxp1 and Satb2, while interneurons in the same clone co-express Sp8 and calretinin.Thus, our preliminary results suggest that, during marine forebrain development, at least some projection neurons and interneurons share single multipotent progenitors.