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The toxic effects to microorganism induced by nanomaterials have received considerable attentions in the past decades [1]. Herein, two diverse nanomaterials i.e. multi-walled carbon nanotubes (MWCNTs) and mesoporous silica nanospheres (MSNs) were prepared to investigate their deleterious effects on Caenorhabditis. elegans (C. elegans)[2-3]. As shown in Figure 1A, histidine functionalized MWCNTs (his-MWCNTs) were in length of ~500 nm with outer diameter ~20 nm, while fluorescein isothiocyanate dyed MSNs (FITC-MSNs) were in an average diameter of ~70 nm (Figure 1B). Microscopic images display his-MWCNTs having been ingested into intestine of C. elegans after co-incubation for 2 h, as arrowed in Figure 1C and 1E. In contrast, no MSNs were observed to be ingested after co-incubating in the same liquid medium. However, fluorescence microscopic images (Figure 1D and 1F) demonstrate that FITC-MSNs could be ingested by C. elegans after co-incubation for 24 h or longer time via seeding Kingagar plates with FITC-MSNs.
The toxic effects to microorganism induced by nanomaterials have been added been attentions in the past decades [1]. Herein, two diverse nanomaterials ie multi-walled carbon nanotubes (MWCNTs) and mesoporous silica nanospheres (MSNs) were prepared to investigate their deleterious effects on Caenorhabditis 1A, histidine functionalized MWCNTs (his-MWCNTs) were in length of ~ 500 nm with outer diameter ~ 20 nm, while fluorescein isothiocyanate dyed MSNs (FITC-MSNs ) were in an average diameter of ~ 70 nm (Figure IB). Microscopic images display his-MWCNTs having been ingested into intestine of C. elegans after co-incubation for 2 h, as arrowed in Figures 1C and 1E. In contrast, no However, the fluorescence microscopic images (Figure 1D and 1F) demonstrate that FITC-MSNs could be ingested by C. elegans after co-incubation for 24 h or longer time via seeding Kingagar plates with FITC-MSNs.