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Ammonia is metabolic waste in most of teleost fish but too much ammonia surrounded is detrimental to fish.Toxicity and effect of NH2-N metabolization of different concentration of ammonia (0 mmol 1-1, 0.1 mmol 1-1, 0.3 mmol 1-1, 0.6 mmol 1-1, 1.1 mmol 1-1 NH4Cl) to juvenile marine medaka were investigated after chronic exposure (2+ months).High concentration of ammonia inhibited feed intake rate and decreased specific growth rate (SGR) of the fish.The ammonia excretion rate was about 10 times higher than the urea excretion rate which indicates that fish mainly excrete ammonia as nitrogenous waste.Ammonia accumulated in fish increased with ambient ammonia during the exposure period but it decreased with time progressed at the same group.