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Nowadays the health of beef in scaledbovine livestock cultivation is a public health issue.It is well know that the intestinal tract houses one of the richest and most complex microbial population on the planet,and a wide range of disease are related to gut dysbiosis or overgrowth of an individual pathogen.In this limited study,the next generation sequencing technologies (NGS) was used to analyze the fecal microbiota structure in healthy beef with different age with different age.