【摘 要】
：
4 kinds of PNIPAAm grafted silica with variable grafting ratio under the same grafting density were prepared via SI-ATRP.The grafting density of ATRP-initiator was regulated through the feed ratio of
【机 构】
：
The school of life science,Beijing institute of technology,Beijing 100081,China
论文部分内容阅读
4 kinds of PNIPAAm grafted silica with variable grafting ratio under the same grafting density were prepared via SI-ATRP.The grafting density of ATRP-initiator was regulated through the feed ratio of acetic chloride to 2-bromoisobutyryl bromide.The chemical group of the stationary phase was assayed by a Fourier transform infrared spectroscopy(FT-IR)method and the content of element C 、H 、N was measured by elemental analyzer.The grafting ratio was measured by Thermogravimetric analysis(TGA).The rentention behavior of 4 kinds of steroids under different temperature was studied.Furthermore,lnk of 10 kinds of compounds with different hydrophobicity was studied under 30 °C and 35 °C.The results indicated that grafting ratio under the same grafting density had a significant influence on the separation effiency.With the increase of grafting ratio,thermosensitivity was enhanced,peak width was increased,and elution time shortened.So it is critical that best grafting rate of temperature sensitive chromatographic materials to separation.
其他文献
近三十多年来,随着经济社会快速发展,工业化和城市化水平不断提高,珠三角地区以O3和PM2.5为代表的大气复合污染形势严峻,此特征在秋季尤为显著[1].较强的大气氧化性是导致秋季大气复合污染频发的重要因素,大气OH自由基是大气氧化性的重要表征.
异丁醇作为一种重要的化学品和燃料添加剂，越来越受到人们的关注。当前异丁醇主要来自于丙烯羰基化制丁/辛醇的副产。煤基合成气合成异丁醇是适合我国能源格局的异丁醇合成路线。尽管合成气制低碳醇研究工作较多，但针对异丁醇合成的研究报道较少。
ZSM-5 分子筛具有独特的孔道结构，是典型的择形催化材料，可通过反应物形选、产物形选、过渡态形选或交通控制形选机制，实现特定目标产物选择性的提升。如何更为充分地利用分子筛的择形本质实现催化过程所追求的高效、高选择性，成为了相关领域关注热点之一。
Glycoproteins play essential roles in many biological processes,such as molecularrecognition,inter-and intracellular signaling,immune response and so on.In addition,many glycoproteins are used as biom
目的：研究异丙醇3浓度对盐酸哌唑嗪与固定化α1A-adrenoceptor(α1A-AR) 相互作用的影响。方法：转染培养HEK293 细胞(Human Embryonic Kidney 293 cell),分离纯化α1A-AR,制备固定化α1A-AR 色谱柱,通过α1A-AR 特异性配体在色谱柱上的保留行为表征色谱柱,使用固定化α1A-AR 色谱柱研究异丙醇浓度对盐酸哌唑嗪与固定化α1A-AR的
癌症是一种多因素疾病,单一药物的治疗效果不明显.因此,具有不同作用机制的多药物联合疗法逐渐受到了人们的重视,然而,药物水溶性差、肿瘤选择性低等缺点依然存在于联合疗法中.本课题组以疏水抗癌药物模型,制备了一类小分子mPEG 修饰的两亲性抗癌潜药,进一步利用mPEG与α-CD 之间的主客体识别作用制备了一系列功能化多聚(准)轮烷(PPR)水凝胶.该类超分子水凝胶可进一步负载和疏水抗癌药物具有协同抗癌活
血浆是临床医学中最为普遍的检测样本,因而成为目前蛋白质组学研究的一个热点对象[1].血浆样品中蛋白质浓度变化范围达到10 个数量级,22种高丰度蛋白质占整个蛋白质组总量的99 ％,中低丰度蛋白质的比例仅为1 ％[2].
N-linked protein glycosylation is the most common post-translational modification(PTM).However,characterizing protein glycosylation is difficult for mass spectrometry(MS)because of low abundance of gl
建立基于化学特征成分和活性相互关联的系统方法,确定藏木香中与抗炎活性具有相关性的药效成分,对来自于不同产地的27个藏木香样品进行分类学鉴定.采用主成分分析(PCA)[1]和聚类分析(HCA) [2]法评价藏木香的HPLC指纹图谱,构建其指纹图谱和抗炎活性的谱效关联模型,通过偏最小二乘法(PLS) 进行相关性研究.
A new thermosensitive chromatographic materials,poly(N-isopropylacrylam-ide-co-2-(diethylamino)ethylmethacrylate-co-N-tert-butylacrylam ide) [poly(NIPAAm-co--DEAEMA-co-tBAAm)] grafted silicafeaturing