Fibroblast-like synoviocytes from adjuvant arthritis rat were transfected with plasmid over-expresse

• 作 者: ShiChunyan;HeQiong;YangJingjing;ZhuangGuohong;
• 机 构: Anti-Cancer Research Center,Medical College,Xiamen University
• 出 处: 中国病理生理学会第十四届肿瘤专业委员会、第十五届免疫专业委员会联合学术会议
• 发表日期: 2014年期

　　Objective Fibroblast-like synoviocytes from adjuvant arthritis (AA) rat were transfected with plasmid over-expressed TIPE2+/+,then apoptosis mediated by TIPE2 on DR5 and its pathological role in Adjuvant Arthritis (AA) were studied in vitro.Methods The plasmid of MIGR1/TIPE2+/+ were transfected to AA-FLS by liposome,after 72 h,the expression of GFP gene that plasmid owned was identified by fluorescence microscope in confirm transfection efficiency,TIPE2mRNA expression was detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR),TIPE2 protein expression of AA-FLS were detected by Western Blot.The growth resistance of cells mediated by ZF1 (DR5 single chain antibody) was detected by MTT method and flow cytometry.Results Plasmid over-expressed TIPE2+/+were 2ansfected to AA-FLS successfully as the fluorescence intensity reached 90%,TIPE2 mRNA and protein expression were significantly improved.Furthermore,the growth inhibition and apoptosis induction effect on FLS/TIPE2 + / + were significant,compared with FLS without processing.Conclusion TIPE2 expression level in AA-FLS were significantly improved by transfecting plasmid expressed TIPE2 + / +,TIPE2 plays an important pathological role in apoptosis mediated by DR5.