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Purpose: Dentin is one of the main components of teeth and has been used as an active scaffold to accommodate odontogenic cells.However,although allogenic dentin has a low immunogenicity,its biological molecules are at risk of being damaged during storage and transportation.In this study,we developed a modified freeze drying technique to prepare a freeze-dried dentin matrix(FDDM)that preserves the bioactivity of dentin but minimizes the immune responses of outside cells.