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Excess dietary intake of nitrates and nitrites has been associated with methaemoglobinaemia and the in vivo production of the carcinogenic N-nitrosamines.Newborn infants are especially susceptible to the detrimental effects of these contaminants, and the regulatory upper limits applied to the production of milk-based infant formulas are generally as low as 2 mg/kg for nitrite and 30 mg/kg for nitrate in national food safety standards of China.Many current methods for the determination of nitrite and nitrate in milk and milk products rely on manual, segmented-flow or flow-injection analysis via cadmium reduction, which is time consuming and poisonous to environment.In most food samples, free amino acids in sample matrix may affect the accurate detection of nitrite and nitrate.