EFFECTS OF CHRYSIN AND ITS TETRAETHYL BIS-PHOSPHORIC ESTER IN THE CELL CYCLE AND P21/WAF1 PROTEINS O

• 作 者: TingZhang[1]XiaolanChen[2]LingboQu[2]ManjiSun[3]YufenZhao[4]
• 机 构: Key Laboratory of Chemical Biology,Department of Chemistry,Zhengzhou University,Zhengzhou,450052
• 出 处: 第17届国际磷化学学术会议(The 17th International Conference on Phosphoru
• 发表日期: 2007年期

　　Aim:To investigate the influences of CR/CP on the cell cycle and p21WAF1 expression in the human cervical carcinoma cell line (Hela).Methods:human cervical carcinoma cell line (Hela) was treated with 10μM CR and CP respectively,the cell cycle and p21WAF1 expression was determined with flow cytometry (FCM) and western immunobloting methods.Results:The cell cycle distribution and p21WAF1 expression in the Hela cells treated with CR/CP were changed markedly as compared to vehicle-treated Control.The CR treatment resulted in an appreciable increase in the G0/G1 phase cell population and a decrease in the S phase cell populations,accompanied by up-regulation of p21WAF1 expression.The CP could increase both in the G0/G1 phase and G2/M phase cell populations,and induce p21/WAF1 expression.Conclusion:CP could arrest cell cycle in both G0/G1 and G2/M phases accompanied with up-regulation p21/wAF1expression,whereas CR only arrest G0/G1 phase cell cycle and induct p21/WAF1 in Hela cells.This indicated that the CR and CP could arrest cell cycle of Hela cells in different ways.