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Hepatic ischemia reperfusion (I/R) injury related to abnormal increased leukotriene (LT) C4 production.Ischemic preconditioning (IP) is shown as a promising technique to prevent liver from the development of I/R injury.In this experiment we investigated whether IP protection roles during hepatic I/R injury might associate with the LTC4 generation in rats.Adult male SD rats were randomly divided into sham, IR and IP groups.Rat liver was subjectcd to 60 min of partial hepatic ischemia followed by 5 h of reperfusion with saline administered intravenously.The LTC4 content was measured by RP-HPLC.Tissue injuries were assessed using serum ALT and AST activities and histological changes.It was observed that compared with I/R group, IP significantly reduced LTC4 content in rat liver (P<0.05).Although serum ALT and AST activities in I/R and IP groups were all higher than those in control group (P <0.01;P<0.001, P<0.01 and <0.05, respectively).The ALT and AST acitvities(P<0.05) in IP groups were markedly lower than those in I/R group.The rat liver tissue in IP group displayed less structure damage than that in I/R group.These results that the degression of LTC4 content resulted from IP treatment accompanied by hepatic ALT and AST decrease as well as lightened liver tissue structure damage, firstly suggest that the protective effects of IP on hepatic IR injury may be partially related to its potential of reducing LTC4 production.