【摘 要】
：
The formation of triple complexes of DNA-liposome-multivalent cations is accompanied by the aggregation and partial fusion of liposomes as we showed by free
【机 构】
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Institute of Biophysics of Cell of Russian Academy of Sciences, Russia
【出 处】
：
2005 World DNA and Genome Day(2005中国(大连)国际DNA和基因组节大会)
论文部分内容阅读
The formation of triple complexes of DNA-liposome-multivalent cations is accompanied by the aggregation and partial fusion of liposomes as we showed by freeze-etching technique.DNA acts as a fusogen in this process,and it unwinds in the region of liposomes fusion.The addition of nuclease S 1 to the triple complexes caused a complete fusion of liposomes and the formation of giant liposomes (1-10 mkm in diameter).The addition of DNA,phosphatidylcholine liposomes,and Mg2+ ions to an egg extract of Xenopus laevis lead to the formation in liposomes of structures resembling nuclear pores but of a smaller diameter.Similar structures was shown in liposomes after staining of triple complexes with terbium or lanthanum.By electron microscopy (freeze-etching technique and by Tb3+ and La3+ staining of triple complexes) we have showed partial fusion of liposomes area,associate with untwisting DNA regions.
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