Effects of 5F on cytotoxicity,apoptosis and cell cycle in human liver cancer cells

• 作 者: YEHua[1]ZHENXue-bao[2]LILi[3]LVYing-nian[3]LIANGNian-ci[4]
• 机 构: Guangdong Key Laboratory for Research and Development of Natural Drugs,Guangdong Medical College,Zha
• 出 处: 2012医学科学前沿暨第二届个体化治疗与抗肿瘤药物研究新趋向研讨会
• 发表日期: 2012年期

　　Objecive:To investigate the anticancer effect of ent-11 α-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F),a compound isolated from Pteris semipinnata L (PsL),in human liver cancer Hep3B cells.Methods:Hep3B cells were treated with 5F (0-80 μg/ml) for different time periods.Cytotoxicity was examined using a MTT method.Apoptosis was examined using Annexin V-EGFP staining and caspase-3 activity.Cell cycle was examined using propidium iodide staining.