【摘 要】
：
蛹虫草是一种寄生于鳞翅目昆虫蛹及幼虫,具有与冬虫夏草相似的活性成分和医疗保健功效的虫草属真菌,我国已经进入产业化生产阶段,但蛹虫草菌种的退化仍然是国内外批量生产的核心技术难题.本文通过研究发现蛹虫草子实体生产性能退化的菌株,是由于不同交配型基因菌株,比例失调或缺失导致的,通过将营养亲和性的mat1-1 和mat1-2 型菌株按照一定比例混合可以使生产性能退化菌株恢复高产性状,并且进一步研究发现,通
【机 构】
：
安徽农业大学,安徽省微生物防治重点实验室,安徽合肥230036;徐州工程学院,食品工程学院,江苏徐州221000
【出 处】
：
第六届全国农业微生物研究及产业化研讨会、第十五届全国杀虫微生物学术研讨会、第十一届全国虫生真菌学术研讨会
论文部分内容阅读
蛹虫草是一种寄生于鳞翅目昆虫蛹及幼虫,具有与冬虫夏草相似的活性成分和医疗保健功效的虫草属真菌,我国已经进入产业化生产阶段,但蛹虫草菌种的退化仍然是国内外批量生产的核心技术难题.本文通过研究发现蛹虫草子实体生产性能退化的菌株,是由于不同交配型基因菌株,比例失调或缺失导致的,通过将营养亲和性的mat1-1 和mat1-2 型菌株按照一定比例混合可以使生产性能退化菌株恢复高产性状,并且进一步研究发现,通过对接两种交配型单孢子菌株自然混合的菌株具有稳定的生产性能,进一步研究发现蛹虫草子实体多糖具有一定的抗氧化和抗肿瘤活性,蛹虫草子实体提取物也具有一定的抑菌活性.研究发现在蛹虫草子实体培养后期,提高温度和增加光照强度,可显著提高蛹虫草子实体多糖和尿嘧啶、尿苷、腺嘌呤、腺苷和虫草素等核苷类物质的含量(P＜0.05),尤其是多糖、腺苷和虫草素的含量较常规培养子实体分别提高0.77、1.76 倍和3.58 倍.另外发现经过高温和强光胁迫蛹虫草子实体抑制酪氨酸酶活性也有所增强,并且从子实体甲醇提取物中分离到酪氨酸酶抑制活性纯品物质,结果显示该物质对单酚酶活性的IC50 为27.8386μg/ml,二酚酶活性的IC50 为38.1432μg/ml.
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