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Applying MS to structural and analytical problems has become increasingly common over last few years, and ESI-MS has been used as and efficient and fast method with direct insertion characterize, confirm and determine constituents in a variety of plant extracts.UPLC takes full advantage of chromatographic principles to perform separations using columns packed with smaller particles (1.7 μm) and/or at higher flow rates resulting in a shorter analysis time, with superior peak capacity (number of peaks resolved per unit time in gradient separations) and sensitivity.