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Objective Selecting and optimizing the prescription of Borneol and Brucea javanica oil nanoemulsion (BBNE), and to prepare the BBNE, then to investigate its tumor suppression effect on glioma in rats.Methods Establishing the GC analysis methods to determinate BBNE content by pre-column derivatization; applying the techniques of phase inversion to prepare BBNE; selecting and optimizing the prescription of BBNE by pseudo-ternary phase diagrams; using transmission electron microscope and laser granulometer to investigate the appearance shape、 determination of particle size distribution and Zeta potential; building glioma tumor model in rats, and investigate BBNE tumor suppression effect by the index of tumor weight and volume.Result Establishing the GC analysis method to determinate BBNE content successfully, selecting and optimizing the best prescription of BBNE is oil phase IPM 35% and Brucea javanica oil (containing 1% Borneol) 35% and emulsifier Cremopher RH 18% and Labrasol 12%; the appearance of nanoemulsion is round and spherical, the average particle size is (47.60±0.57) nm, the PDI is (0.22-0.01), the Zeta potential is (-1.06±0.12) mV, and the drug loading capacity is (0.4241±0.0056) mg·mL-1; tumor inhibition experiments show that the tumor weight and volume of Saline injection group、 Brucea javanica oil injection group、 Brucea javanica oil nanoemulsion group and BBNE group is (1.32±0.19)、 (0.84±0.08)、 (0.76±0.06)、 (0.57±0.10)g and (72.2±9.4)、 (44.2±5.1)、 (42.3±4.9)、 (27.9±2.5) mmn3 respectively, and the tumor inhibition rate is 36.49%, 42.80%, 56.94%.Conclusion The prescription is very well because the BBNE has uniform particle size distribution、 higher drug content and better stability, and that BBNE has better tumor suppression effect on glioma in rat show Borneol can promote Brucea javanica oil across the blood-brain barrier to improve tumor suppression effect preliminarily.