【摘 要】
：
[Objective ]The objectives of this study are to clone goat PHLDA2(pleckstrin homology-like domain,family A,member 2),to analyze its tissue expression and imprinting status,and to reveal its relationsh
【机 构】
：
Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization,College of
【出 处】
：
中国”西南地区农业动物种质资源挖掘与创新利用“学术研讨会
论文部分内容阅读
[Objective ]The objectives of this study are to clone goat PHLDA2(pleckstrin homology-like domain,family A,member 2),to analyze its tissue expression and imprinting status,and to reveal its relationship with placental traits,thereby providing data for further investigation on the gene functions in goat placenta.[Method]Partial cDNA sequence of PHLDA2 gene was cloned for Dazu black goats according to the conserved regions among human,cattle and pig sequences,and the full-length cDNA of this gene was further cloned with 5-and 3-RACE.Sequence characteristics of goat PHLDA2 were analyzed using online softwares,such as ORF Finder and ProtParam.Real-time PCR was applied to exam tissue expression of goat PHLDA2 in 10 tissues,including heart,liver,spleen,kidney,skeletal muscle,lung,tongue,fat,brain and placenta.Imprinting status of the gene in placenta was investigated by expressed SNP method.
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