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Our purpose is to obtain gradient estimates for certain nonlinear partial differential equations by coupling methods.First we derive uniform gradient estimates for a certain semi-linear PDEs based on the coupling method introduced in Wang(2011)and the theory of backward SDEs.Then we generalize Wangs coupling to the G-expectation space and obtain gradient estimates for nonlinear diffusion semigroups,which correspond to the solutions of a certain fully nonlinear PDEs.