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目的:建立离子色谱法(IC),同时测定高热值混合糖电解质注射液中6种阴离子(葡萄糖酸根、醋酸根、氯离子、硫酸根、磷酸根和枸橼酸根)及4种阳离子(钠离子、钾离子、镁离子和钙离子)的含量方法;方法:采用离子色谱仪,电导检测器检测;测定阴离子的色谱柱为IonPac AS23 column(250 mm×4 mm),流动相为氢氧化钾溶液,按浓度梯度洗脱(KOH浓度:0～8min为5 mmol.L-1,8.1～25 min为35 mmol.L-1,25.1～30 min为5 mmol.L-1);测定阳离子的色谱柱为IonPac SCS1 col-umn(250 mm×4 mm),流动相为6 mmol.L-1甲烷磺酸溶液。结果:本方法可在同台离子色谱仪上分别使用阴阳离子系统分析产品中各阴阳离子含量,各成分间分离良好,各成分的浓度和各自峰面积之间有着良好的线性关系,精密度及加样回收率的RSD均小于2.0%。结论:本方法快捷、准确、专属、精密、耐用,可较全面地控制产品质量。
Objective: To establish an ion chromatography (IC) method for simultaneous determination of six anions (gluconate, acetate, chloride, sulfate, phosphate and citrate) and four cations , Potassium ion, magnesium ion and calcium ion). Methods: Ion chromatography and conductivity detector were used for detection. The anion was measured on an IonPac AS23 column (250 mm × 4 mm) with a mobile phase of potassium hydroxide solution , According to the concentration gradient (KOH concentration: 0 ~ 8min 5mmol.L-1,8.1 ~ 25min 35mmol.L-1,25.1 ~ 30min 5mmol.L-1); Determination of cation chromatography The column was IonPac SCS1 col-umn (250 mm × 4 mm) and the mobile phase was 6 mmol·L-1 methane sulfonic acid solution. Results: In this method, anion and cation systems were used to analyze the content of each anion and cation in the ion chromatography on the same platform. The separation between each component was good. There was a good linear relationship between the concentration of each component and the peak area, The RSDs of recoveries were less than 2.0%. Conclusion: The method is fast, accurate, exclusive, precise and durable, which can control product quality more completely.