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I-HmuI and I-Basl are two highly similar homing endonucleases,each encoded by a group I intron inserted into homologous sites within the DNA polymerase genes of Bacillus phages SPO1 and Bastille.These proteins are under intense scrutiny for a variety of applications,as they are highly specific DNA nicking enzymes.I-BasI has properties that are typical of homing endonucleases,nicking only the intron-minus polymerase gene in either host genome.In contrast,I-HmuI nicks both the intron-plus and intron-minus site in its own host genome,but does not act on the target from Bastille phage.