A comparison between two methods of myelin staining in cuprizone-induced demyelination model mice

• 作 者: LiZHANG;Lin-LinYIN;LinLI;
• 机 构: Department of Pharmacology,Xuanwu Hospital of Capital Medical University
• 出 处: 第二届世界天然药物和传统药物药理学学术会议
• 发表日期: 2012年期

　　AIM To compare two methods of myelin staining for detecting the morphological changes in the brain of cuprizoneinduced demyelination model mice.METHODS Demyelination model was induced by cuprizone which was mixed in forage and fed to mice for 4 weeks.After the brain tissue sections were made, Luxol Fast Blue (LFB) and oil red O staining methods were used to observed demyelination changes in the corpus callosum on striatum and hippocampus sections.RESULTS The results from both LFB and oil red O staining showed that demyelination occurred in the corpus callosum of cuprizome-induced model mice, but the morphological changes in the striatum section were clearer detected by oil red O staining than that measured by LFB staining, and the margin of demyelination with the surrounding tissue was not clear on the section stained by LFB.CONCLUSION Compared with the LFB staining, oil red O staining shows clearer demyelination and is a simple myelin staining method.