【摘 要】
：
Objectives:To investigate the expression of prolyl 4-hydroxylase(P4HB)in lung carcinoma and the intervention effect of Yiqi Chutan Formula(YQCTF).Methods:Lung carcinoma model was established by subcut
【机 构】
：
Department of the Clinical Teaching and Research College of Traditional Chinese Medicine Chongqing M
论文部分内容阅读
Objectives:To investigate the expression of prolyl 4-hydroxylase(P4HB)in lung carcinoma and the intervention effect of Yiqi Chutan Formula(YQCTF).Methods:Lung carcinoma model was established by subcutaneously inoculating LEWIS lung carcinoma cells in C57BL/6J mice.The expressed differentially protein spots information of YQCTF(3.0 g/kg/d,gavage,daily,21 days)and model group were acquired by a two-way fluorescence difference gelelectrophoresis(2D-DIGE),and identified by matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF/TOF-MS).The differences in protein expression of P4HB was verified by Western blotting.Test the expression of P4HB by the application of clinical pathology and tissue microarray to obtain the clinical validation.Cultured A549 cells,divided into 15%serum containing of YQCTF and 15%serum combined DDP 1.5μg/ml group,cellular immunohistochemistry and RT-PCR detected the expression of P4HB and P4HBmRNA.Results:The proteomics research discovered that one-third of differential proteins which include P4HB were unfolded protein response(UPR)or related proteins were decreased in the YQCTF group(p<0.01).Clinical pathology and tissue microarray studies showed that P4HB expression in lung cancer tissue was stronger than adjacent tissues and normal lung epithelial(p<0.01).In the YQCTF and DDP combined group,the expression of P4HB and P4HBmRNA of A549 cell were decreased significantly(p <0.01).Conclusion:YQCTF could inhibit the LEWIS lung carcinomas growth,decrease the expression of P4HB in LEWIS lung carcinoma and A549 cells.YQCTF might take effect through regulating UPR in endoplasmic reticulum to inhibit the incidence and growth process of lung carcinoma.
其他文献
目的:从阴道局部免疫角度探讨完带汤治疗外阴阴道假丝酵母菌病(RVVC)的作用机制.方法:采用病例对照研究方法,将符合纳入标准的RVVC 患者随机分为中药完带汤巩固治疗组及西药伊曲康唑巩固治疗组,各30例,另选取23例健康妇女作正常对照组,均巩固治疗6个月并于治疗结束后随访6个月.巩固治疗第1、3、6个月月经干净后及随访第1、3、6个月月经干净后复查阴道分泌物并填写临床观察表,进行疗效评价.并于RV
目的:初步探讨盆腔炎性疾病后遗症(慢性盆腔痛)的中医证型分布规律、各证型的常见症状分布特征及相关因素,为形成规范、客观的辨病辨证论治提供新思路,为该病临床辨证论治和科学研究提供流行病学依据.方法:采用回顾性、面访式调查研究挖掘流行病学资料方法,收集从2012-2014年的盆腔炎性疾病后遗症(慢性盆腔痛)患者的中医证候四诊资料,揭示该病中医证型分布规律及各证型常见症状的分布;并分析年龄、病程、职业等
目的:介绍中医药治疗胃癌合并高凝状态的临床试验方案,并评价方案的设计特色.方法:以晚期胃癌合并高凝状态患者为研究对象,采用多中心、前瞻性、实用性随机对照试验方法,通过规范临床试验,客观评价中医药治疗胃癌合并高凝状态的临床疗效,通过分析相关指标,探索运用益气活血理论治疗晚期胃癌合并高凝状态的临床机制.结果:运用中医“气虚血瘀”理论,结合现代实验室相关指标,共同判断中医药治疗胃癌合并高凝状态的临床疗效
目的:观察莲龙消积方联合榄香烯介入治疗对中晚期原发性肝癌(PHC)患者的临床疗效.方法:采用随机对照临床试验方法,将55例中晚期PHC患者分为治疗组25例,对照组30例,对照组行常规TACE,治疗组在对照组基础上加用榄香烯灌注或栓塞治疗,并口服莲龙消积方.结果:治疗组和对照组的实体瘤疗效有效率分别为20.00％和13.33％,临床获益率为96.00％和83.33％;治疗组治疗后ALB、ALT、AS
目的:探讨中药保留灌肠加理疗治疗输卵管炎性阻塞性不孕症的临床疗效;不同给药途径的疗效比较.方法:治疗组给予自拟抗炎通管汤中药保留灌肠配合理疗,对照组给予金鸡胶囊口服配合理疗.结果:治疗组痊愈率和好转率均优于对照组.结论:中药保留灌肠加理疗是治疗输卵管炎性阻塞性不孕症的有效方法.抗炎通管汤疗效优于金鸡胶囊.
目的:探讨晚期非小细胞肺癌(NSCLC)患者的中医体质特征对应用吉非替尼后的无进展生存期(PFS)的影响.方法:2013年10月-2015年3月期间,在中国中医科学院广安门医院肿瘤科门诊就诊及病房住院采用吉非替尼治疗的111例NSCLC的患者进行回顾性分析.结果:在所有患者样本分析中,患者总体的PFS为10.0个月(2.0-22.0个月),其中阳虚质14.0个月(8.0-22.0个月)与平和质11
目的:观察健脾解毒方治疗转移性大肠癌的有效性和安全性.方法:入选220例转移性大肠癌患者按1:1随机分为治疗组和对照组,两组均接受FOLFIRI、XELOX、FOLOX方案化疗,治疗组在化疗基础上加用中药健脾解毒方.比较两组临床客观疗效、生存情况、生活质量、不良反应.结果:治疗组脱落15例,对照组脱落23例,治疗组可评价95例患者中无完全缓解,部分缓解29例,稳定41例,客观有效率(RR)33.3
为探究中药复方消岩汤抗肿瘤效应物质基础,本文采用整方研究策略,以体外免疫调节、细胞毒、促细胞凋亡等抗肿瘤相关的活性为指导,运用HPLC法,以与肿瘤密切相关的多个靶点,探讨复方消岩汤抗肿瘤作用的效应成分,后进行成分标准品比对,以进一步验证成分.结果显示运用HPLC-MS法测定复方消岩汤剂的乙腈相中分离得到约55个单体化合物,鉴定了5个化合物的结构,包括皂苷类、五环三萜类、二酮类、醇酯类等4种结构类型
目的:探讨不同分期原发性肝癌的中医证候分布规律.方法:对符合纳入条件的559例肝癌患者进行中医四诊资料的采集,根据本单位制定的诊断规范进行证候判定,获取患者的基本证候量化积分,从定性和定量两方面观察不同临床分期肝癌患者的证候分布,并结合聚类分析初步探讨其证候组合规律.结果:(1)比较证候在各期的分布及量化积分情况,肝癌患者的基本证候中血瘀证和气虚证最常见,气滞证在Ⅰ期的分布和积分多于Ⅱ、Ⅲ期;水湿
目的:分析总结盆腔炎性疾病后遗症(湿热瘀结型)采用中医综合治疗方案治疗的临床方法及疗效.方法:纳入510例湿热瘀结型盆腔炎性疾病后遗症患者,采用中医综合治疗方案(二联疗法及三联疗法),观察并分析不同治疗方案的临床疗效.结果:510 例湿热瘀结型盆腔炎性疾病后遗症患者经过中医综合疗法治疗后,缓解盆腔疼痛愈显率73.14％-78.52％,改善局部体征愈显率59.92％-66.67％,消散盆腔包块愈显率