【摘 要】
：
We reported a simple laser-induced Rayleight light scattering (LIRLS) for ultrasensitive detection of Hg2+ and K+ ions based on the use of aptamer as a recognition element and gold nanoparticle as a r
【机 构】
：
Department of Chemistry,National Sun Yat-sen University,Taiwan
【出 处】
：
第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
论文部分内容阅读
We reported a simple laser-induced Rayleight light scattering (LIRLS) for ultrasensitive detection of Hg2+ and K+ ions based on the use of aptamer as a recognition element and gold nanoparticle as a reported element.Because laser-induced light scattering of the AuNPs increased with increasing the particle size,aggregated AuNPs exhibited relatively strong Rayleight scattering relative to dispersed AuNPs.Based on thais concept,LIRLS is a suitable strategy to combine salt-induced gold nanoparticle sensor.We utilized the 488 or532 nm laser to excite directly the cuvette containing gold nanoparticle and collected Rayleight scattering signal by the portable spectrometer which placed in 90°to isolate the intense laser beam and Rayleight scattering signal.We modified the gold nanoparticles with polythymine oligonucleotides T33 for selective detection Hg2+ ions and K+ aptamer (GGG TTA GGG TTA GGG TTA GGG) for selective detection K+ ions.Gold nanoparticles adsorbed on randomly oligonucleotides were dispersed well in PBS because of strong electrostatic repulsion between DNA molecules.The formation of T-Hg2+-T with Hg2+ ions and four-stranded tetraplex structure (G-quartet) complexes with K+ ions enabled the removal of oligonucleotides from the gold nanoparticles surface,resulting in salt-induced gold nanoparticles aggregation.The average diameter of aggregated gold nanoparticle increased linearly with the concentration of targets.Under optimum conditions,the average diameter of above modified nanoparticle increased linearly with the target Hg2+ concentration over the range from 0.1 nM to 100 nM,with a detection limit of 0.05 nM and K+ concentration ranging from 50 μM to 10 mM,with a detection limit of 1 μM.The result shows that the combination of salt-induced gold nanoparticle aggregation and laser-induced rayleight light scattering method has great potential in the analysis of interesting analyst.
其他文献
Carbon based nanomaterials such as carbon nanotube (CNT) and carbon nanofiber (CNF) [1] have been studied for application to fuel cells,capacitors and various biosensors.We have greatly improved the d
Objectives: Squamous cell carcinoma (SCC) and salivary gland adenoid cystic carcinoma (ACC) are two common malignancies in the oral and maxilla-facial region[1,2].It has been proved that angiogenesis
We reported the development of two- and three-input logic devices based on the fact that Hg2+-,Ag+-,and/or coralyne can drive fluorescence quenching of oligonucleotide-based probe.The designed probe i
Chronic myeloid leukemia (CML) is caused by BCR/ABL,resulting from reciprocal translocation between chromosomes 9 and 22,t (9;22)(q34;q11).1 BCR/ABL is a specific marker of the disease sharing a high
Chitosan is an abundant,biocompatible and biodegradable biomaterial,and its degradation products are harmless natural metabolites,and non-antigenic.Chitosan is an amino-polysaccharide that under moder
Electrochemical impedance spectroscopy (EIS) analysis can detect an analyte with high sensitivity in short time.The self-assembled monolayer (SAM) technique is usually used for attaching analytes to a
Probe antibody immobilization on matrix surface plays a key role in solid phase immunoassays.The traditional amide bond formation by EDC/NHS chemistry often leads to random orientation as it binds the
This paper introduces a nano-CEEC chip incorporating nano-electrodes with sequential applied electric fields to pre-concentrate scarce neurotransmitters released from PC-12 cells for continuous detect
Efficient single cell encapsulation and long term culture are important for many single cell studies.Droplet microfluidics enables compartmentalizing massive single cells into monodipersed droplets.[1
This study developed an adenosine-based molecular beacon (MB) for selective and sensitive detection of heparin at room temperature based on the fact that electrostatic attraction between heparin and c