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Objectives: The studies aimed at analysis of cytokine response (TNF-alpha, IL-lbeta, IL-8 and IL-10) in patients with colon cancer.Methods: The studies were performed on 15 adult health volunteers forming a control group (group 1) and on 15 adult patients with colon cancer (group 2).In all cases of group 2 histological evaluation of tumour biopsies disclosed the presence of advanced moderately differentiated adenocarcinoma (G2) of the colon.In parallel, patients of group 2 were subjected to partial resection of the bowel with tumour with reconstruction of the gastrointestinal tract continuity.In post-operative material, no metastases were notedin the lymph nodes, In sera of all examined individuals (in patients ofgroup 2 before surgery) levels of TNF-alpha, IL-lbeta IL-8 and IL-10 were estimated, employing high sensitivity ELISA kits (Quantikine HS, R and D Systems).Results: Sera of the control group (group 1) contained mean levels of TNF-alpha of 0.70±0.49 pg/ml, IL-1 beta of 0.11±0.10 pg/ml, IL-8 of 9.77±7.65 pg/ml, IL-10 of 1.36±0.98 pg/ml.In turn, sera of group 2 patients contained mean levels of TNF-alpha of 1.50±0.91 pg/ml, IL-lbeta of 0.81±0.89 pg/ml, I L-8 of 31.84± 12.97 pg/ml, IL-10 of 10.92±4.29 pg/ml.Statistical analysis disclosed that only IL-10 level was significantly elevated as compared to the level in the control group.Conclusion: In the course of colon cancer, secretion of pro-inflammatory cytokines (TNF-alpha, IL-lbeta, IL-8) seems to be reduced due to regulatory function of IL-10, most probably spontaneously produced by tumour cells.