A NOVEL UNIVERSAL REAL-TIME PCR SYSTEM USING THE ATTACHED UNIVERSAL DUPLEX PROBES FOR QUANTITATIVE A

• 作 者: Litao Yang
• 机 构: Shanghai Jiaotong University, Shanghai 200240, CHINA
• 出 处: 第十四届世界食品科技大会
• 发表日期: 2008年1期

　　Real-time PCR techniques are being widely used for nucleic acids analysis,and one limitation of current mainly employed real-time PCR is the high cost of the labeled probe for each target molecule.One real-time PCR technique employing attached universal duplex probes (AUDP) is developed,which is supposed to have the advantage of generating fluorescence by probe hydrolysis and strand displacement over current real-time PCR methods.AUDP is one set of universal duplex probes in which the 5 end of the fluorescent probe (FP) and a complementary quenching probe (QP) lie in close proximity so that fluorescence can be quenched.