Isolation,identification and partial biological characterization of a border disease virus from shee

• 作 者: Li Mao Xia Liu Wenliang Li Yongqian Zhao Leilei Yang Wenwen Zhang Jieyuan Jiang
• 机 构: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veteri
• 出 处: 中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第十次学术研讨会
• 发表日期: 2014年12期
• 关键词: BDV complete genome sequence phylogenetic analysis experimental infection

　　Border disease virus (BDV) causes border disease (BD) affecting mainly sheep and goats.In this study, one case of BDV infection was detected positively by RT-PCR from one slow-grown sheep in a sheep farm in Jiangsu province of China, and further confirmed by sequencing and alignment.The infected sheep was as one persist infected (PI) animal being BDV RNA positive and negative specific antibody response in the next 6 months of whole grown period.The virus strain was isolated in MDBK cells without cytopathic effect (CPE) and named as JSLS12-01.The phylogenetic trees through alignment of 5-UTR and Npro gene indicated that the isolated virus was belonged to BDV-3 genotype as high homology with the other 3 BDV strains previously isolated from Chinese goats.The obtained genome of JSLS12-01 was 12, 227 nucleotides (nt) in length, including a large open reading frame (ORF) of 11,694 nt for a single polyprotein flanked by 5-(~278nt) and 3-(255nt) UTRs.The sequenced genome of the virus possessed the highest homology (87.7% in nucleotides) with the BDV-3 strain Gifhorn.Initial experimental infections showed that the infected animals were short-period fever (5 days) and viremia confirmed by RT-PCR and virus isolation in 1-7 days post-infection.This report would confirm the occurrence and partial characteristics of BDV strain JSLS12-01 persistent infection in small ruminants in China.