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A combined QM/MM protocol for the simulation of resonance Raman spectra of protein and peptides has been proposed and implemented [1].The divide and conquer strategy was applied to incorporate the localized contributions from peptide bond π-π* excitations and vibronic coupling.A Ramachandran angle map (RAM) was constructed as a vibronic coupling database.Ultrafast one-dimensional (1D) and two-dimensional (2D) stimulated resonance Raman (SRR) techniques were proposed to reveal the vibronic coupling and vibrational energy transfer between different modes,which can be used to detect the local chemical environment of a specified residue.[2]We also applied the 2DSRR technique to characterize the folding states of the Trp-cage mini-protein.[3]