论文部分内容阅读
Rare circulating tumor cells (CTCs) are present in the bloodstream of millions of patients and increasing asymptomatic cancer survivors and are the root cause of cancer metastases and deaths.Current strategies that are used to isolate and characterize CTCs include CellSerach and others.To explore whether other methods can be methodologically more routine,versatile,and successful than the current ones in quantitative isolation,characterization and ex vivo culture of CTCs,we established and validated a novel method that combines immunomagnetic negative enrichment and multiparametric fluorescence-activated cell sorting (FACS) for the propose.