Efficient Analytical Method for Biomolecules Using Hydrogel

• 作 者: MasaruKato;ShuheiMurayama;
• 机 构: Graduate School of Pharmaceutical Sciences and Global COE Program, The University of Tokyo, Tokyo 11
• 出 处: 第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
• 发表日期: 2011年期

　　Proteins have several remarkable advantages over conventional chemical catalysts, including high selectivity and efficiency; absence of undesirable side reactions; operability under mild conditions, such as in water at ambient temperature; and environmental friendliness.For these reasons, proteins are widely used in biotechnology, industry, and clinical trials.However, proteins are denatured by heat and pH changes, are less soluble than some conventional catalysts, and also can suffer from autolytic degradation, all of which result in an overall decrease in the proteins usability.We have developed a hydrogel-based protein immobilization scheme that allows proteins to maintain their activity for a long period of time (more than one year).This hydrogel consists of a hydrophilic polymer swollen by water that is insoluble owing to physical and chemical cross-links and is able to encapsulate proteins without chemically bonding to them.We prepared protein encapsulated hydogels and used for many analytical fields such as chiral separation [1], proteome analysis [2], enzyme assay [3].