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The method high performance thin-layer chromatography (HPTLC) is now actively used for separation and quantitative determination of organic and inorganic compounds in pharmacology, biochemistry, medicine and other areas.Flavins represent an important class of biomolecule.The three main derivatives of flavins include the water-soluble vitamin riboflavin (RF) and its two biologically active metabolites, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD).