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目的:观察老年脓毒症患者外周血单核细胞Toll样受体(TLR)表达谱的改变。方法:37例脓毒症患者,按照年龄分为成年脓毒症组(<60岁,17例)、老年脓毒症组(≥60岁,20例),同时设立健康对照:成年对照组(<60岁,20例)、老年对照组(≥60岁,20例)。流式细胞仪检测外周血单核细胞Toll样受体的表达。结果:老年对照组、成年对照组均以TLR6、TLR8表达最高,两组间无明显差异。成年脓毒症组TLR2[(69.97±29.29)%]、TLR7[(24.73±22.77)%]较成年对照组TLR2[(16.00±18.62)%]、TLR7[(3.92±3.06)%]明显增高(P<0.05)。老年脓毒症组TLR2[(27.44±33.64)%]较老年对照组TLR2[(3.61±5.92)%]明显增高(P<0.05)。老年脓毒症组TLR2[(27.44±33.64)%]、TLR8[(15.30±21.25)%]较成年脓毒症组TLR2[(69.97±29.29)%]、TLR8[(34.75±27.64)%]明显降低(P<0.05),余无明显差异。结论:正常老年人外周血单核细胞Toll样受体的分布与成年人相似,TLR2在脓毒症患者中发挥着重要的作用,脓毒症时老年患者免疫反应较成年患者弱。
Objective: To observe the change of Toll-like receptor (TLR) expression in peripheral blood mononuclear cells in senile sepsis patients. Methods: Thirty-seven sepsis patients were divided into adult sepsis group (<60 years old, 17 cases) and senile sepsis group (≥60 years old, 20 cases) according to their ages. At the same time, healthy control group <60 years old, 20 cases), elderly control group (≥60 years old, 20 cases). Flow cytometry detection of peripheral blood mononuclear cells Toll-like receptor expression. Results: The expression of TLR6 and TLR8 in elderly control group and adult control group were the highest, with no significant difference between the two groups. Compared with adult control group, TLR2 [(69.00 ± 18.62)%] and TLR7 (3.92 ± 3.06%) were significantly higher in adult sepsis group (69.97 ± 29.29%, TLR7 [(24.73 ± 22.77)%] P <0.05). TLR2 [(27.44 ± 33.64)%] in elderly sepsis group was significantly higher than that in elderly control group [(3.61 ± 5.92)%] (P <0.05). Compared with adult sepsis group, TLR2 [(69.97 ± 29.29)%] and TLR8 [(34.75 ± 27.64)%] in TLR2 [(27.44 ± 33.64)%] and Reduce (P <0.05), I no significant difference. CONCLUSIONS: The distribution of Toll-like receptors in peripheral blood mononuclear cells in normal elderly is similar to that in adults. TLR2 plays an important role in sepsis. Elderly patients with sepsis have a weaker immune response than adult patients.