Development of EST-derived SSR markers in Actinidia arguta and genetic diversity analysis

• 作 者: WANG Jiahui SUN Haiyue GUO Ruixue WANG Fawei WANG Ming LI Yadong
• 机 构: CollegeofHorticulture,JilinAgriculturalUniversity,Changchun130118,China
• 出 处: 2013全国植物生物学大会
• 发表日期: 2013年10期
• 关键词: Actinidia arguta EST-SSR genetic diversity analysis

　　The objective of this study was to analyze the SSR distribution in Actinidia and develop new EST-derived SSR markers.A total of 132 593 ESTs of Actinidia were downloaded from the NCBI database and processed to get unique sequences by CAP3 software.SSRIT was used to search SSRs.Subsequently, the unique sequences with minimum 50 bp flanking were used to design EST-SSR primers using Primer 5.0.40 primer pairs that own higher comprehensive score were randomly selected to test by PCR of 36 A.arguta germplasm accessions.