【摘 要】
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Fatty liver is a major metabolic disorder that occurs during early lactation in high-producing dairy cows.Sterol regulatory element-binding protein-1c (SREBP-1c) is an important transcription factor t
【机 构】
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Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University,
【出 处】
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中国畜牧兽医学会兽医内科与临床诊疗学分会第八届代表大会暨学术研讨会
论文部分内容阅读
Fatty liver is a major metabolic disorder that occurs during early lactation in high-producing dairy cows.Sterol regulatory element-binding protein-1c (SREBP-1c) is an important transcription factor that regulates lipid synthesis by regulating the expression of lipid metabolism genes.In this study, we reduced the expression of SREBP-1c by adenovirus-mediated SREBP-1c with a low expression vector (AD-GFP-SREBP-1c) to study the effects of SREBP-1c on lipid deposits in bovine hepatocytes.The expression level and enzyme activity of SERBP-1c and its target genes were detected by real-time PCR, western blot, and ELISA.These results showed that AD-GFP-SREBP-1c could inhibit expression of SREBP-1c.The expression of the lipid synthesis enzyme acetyl-CoA carboxylase (ACC) was down-regulated.The expression levels of the lipid oxidation enzymes long-chain fatty acyl-COA synthetase (ACSL-1), carnitine palmitoyltransferase Π (CPT-Π), and β-hydroxyacyl-CoA-DH (HADH) were significantly elevated.Furthermore, compared with the negative control and the blank control groups, the expression levels of factors involved in the assembly and transport of very low-density lipoproteins (VLDLs), such as apolipoprotein B 100 (ApoB), apolipoprotein E (ApoE), and microsomal triglyceride transfer protein (MTTP) were decreased but the low-density lipoprotein receptor (LDLR) was elevated.The concentrations of TG (triglyceride) and VLDL were also reduced.These data suggest that low expression of SREBP-1c can decrease lipid synthesis,increase lipid oxidation, and decrease the TG and VLDL content in bovine hepatocytes.
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