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The pluripotency of embryonic stem cells (ESCs) is regulated by multiple layers of factors.At the RNA level,we have identified a cluster of miRNAs whose expression abundance is positively correlated with the pluripotency level of ESCs,and confirmed that one of the functions of these miRNAs is to target the key component of the PRC2 complex therefore to regulate H3K27me3 modification.We also identified a long non-coding RNA which functions as a ceRNA to compete for miRNAs targeting a key pluripotency factor,Nanog.We have proven that ESC-specific transcription factors are capable to produce ESC-specific transcripts with alternative transcription start sites from ubiquitously expressed genes,thus confer ubiquitously expressed genes novel functions to involve in the maintenance of ESC pluripotency.In addition,the m6A modification on mRNAs is also required for reprogramming of MEFs to induced pluripotency stem cells (iPSCs).