CHARACTERIZATION OF THREE GENES ENCODING THE SUBUNITS OF LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUC

• 作 者: ChunleiShi;
• 机 构: Department of Food Science and Technology, Shanghai Jiao Tong University, Shanghai, China
• 出 处: 第十四届世界食品科技大会
• 发表日期: 2008年期

　　Light is necessary for hydrogenation of the D ring of protochlorophyllide leading to chlorophyllide formation in higher plants (light-dependent pathway),but it is not essential in phototrophic bacteria (dark pathway).Both pathways,however,occur in some algae,mosses,ferns,and gymnosperms,and each chloroplast genome of these organisms contains three genes,chlL,chlN,and chlB,encoding the three subunits of light-independent protochlorophyllide reductase (LIPOR) required for protochlorophyllide reduction in the dark.In this study,the three LIPOR genes chlL,chlN,and chlB were cloned from the chloroplast of Chlorella protothecoides CS-41 (CSIRO),which grew heterotrophically with considerable chlorophyll yield.