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Miniaturization of fluid handling and fluid analysis is a recent trend in analytical chemistry and life sciences.Microfluidic applications cover micro arrays, DNA sequencing, sample preparation and analysis, cell separation and detection, and environmental monitoring etc.As the integration and functionality of microfluidic devices increase, the precise control of fluid in complex channel network becomes more and more important.A numerical analysis of flow through such network can be achieved using the finite difference method, the finite element method, and the finite volume method.However, there is no efficient way to design such network based on user-defined objectives and constraints.In this presentation, we will reports an effective topology optimization procedure for designing no-moving part microfluidic devices, including Tesla valve, Venturi diode,pump, chaotic mixer, and channel network.