Expression of Nemo-like kinase (NLK) in the brain in a rat experimental subarachnoid haemorrhage mod

• 作 者: GangChen;ZhongWang;SAHResearchTeam;
• 机 构: Department of Neurosurgery, The First Affiliated Hospital of Soochow University
• 出 处: 中华中青年神经外科交流协会首届学术大会
• 发表日期: 2012年期

The present study aimed to investigate the expression of the Nemo-like kinase (NLK) in the brain after experimental subarachnoid haemorrhage (SAH) in rats.A total of 36 rats were randomly divided into six groups:control group; day 1,day 3,day 5,day 7 and day 14 groups.Day 1,day 3,day 5,day 7 and day 14 groups were all SAH groups in which the rats were killed on day 1,3,5,7 and 14,respectively.In SAH groups,autologous arterial blood was injected into cisterna magna once on day 0.Immunostaining and immunoblotting experiments were performed to detect the expression of NLK protein.Real-time polymerase chain reaction (PCR) was used to analyze the presence and quantity of NLK mRNA.The expressions of NLK protein and mRNA were decreased remarkably in SAH groups compared with the control group. The immunohistochemical staining demonstrated expression of NLK was present mainly in the neurons in the brain.Our results indicate that NLK expression is down-regulated in the brain after experimental SAH.