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In order to screen out the antagonistic microorganisms with benign symbiosis and enhance the control effects of tobacco soil-borne diseases by using multiple biocontrol strains,seventeen species of antagonistic microorganisms (mainly included 6 species of Trichoderma,4 species of bacteria and 7 spe-cies of actinomycetes),which were screened and proved that have strong inhibition effects on common tobacco root pathogenic fungus from tobacco growing soil of Luoyang,were used as the research object of this experiment on the basis of previous studies.