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OBJECTIVE To investigate effect of AQP4 knock out on pain sensation.METHODS AND RESULTS Acute pain responses in wild-type mice and in mice lacking AQP4 were compared.AQP4-/-mice had reduced responsiveness to ther mal (hot plate test, hindpaw radiant heat (Hargreaves) test and tail-flick test) , chemical (formalin and capsaicin test) , or me chanical (yon Frey test) stimuli.