Screening of differentially expressed genes in the growth plate of broiler chickens with tibial dysc

• 作 者: Wen-xiaTian[1]Ding-zongGuo[2]Jia-kuiLi[2]PingQin[2]RuiWang[2]Guan-baoNing[3]Jian-gangQiao[3]Hong-quanLi[3]Ding-renBi[4]Si-yiPan[5]
• 机 构: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070 China
• 出 处: 中国畜牧兽医学会第三届中国兽医临床大会
• 发表日期: 2012年期

　　Background The aim of this study was to identify differentially-expressed genes (DEGs) in the growth plate of broiler chickens associated with tibial dyschondroplasia (TD) induced by thiram.RNA was extracted from the growth plates of chickens that consumed control or thiram diets for 1,2,and 6 days.Total RNA were analyzed using eighteen chicken Affymetrix GeneChip assays to identify DEGs.The identified DEGs were also verified by real-time reverse-transcription polymerase chain reaction (RT-PCR).Results The microanalyses identified 880 DEGs,with 108,385,and 468 DEGs exhibiting at least 1.5-fold changes at days 1,2,and 6,respectively.Seven representative DEGs were further verified by real-time RT-PCR and confirmed to be upregulated in TD chickens,including lysyl oxidase (lox),heat shock protein 25 (hsp25),inhibitor of DNA binding 1 (id-1),kinectin 1 (kntl),secreted frizzled-related protein 4 (sfrp4),cadherin 1 (cdh 1),and enolase 2 (eno2).Conclusion Our findings provide a reliable bioinformatics basis for better understanding the molecular pathogenesis of TD.